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JMBS 2019, 4(4): 255–260
https://doi.org/10.26693/jmbs04.04.255
Biology

Some Aspects of Lead Exclusion with Excrements from Laboratory Rats in the Experimental Conditions

Zemlianyi O. A.
Abstract

Contamination of the environment is one of the most important challenges of today’s world. Heavy metals are considered to be the most dangerous environmental contaminants. Lead (Pb) is one of the most toxic elements for living organisms. Chronic intake of lead causes numerous severe irreversible disorders. The purpose of the study was to obtain the results on characteristics of the exclusion of a dangerous toxic element, lead, from the organisms of laboratory rats after its oral administration; to determine the lead amount and concentration excreted from the animal body during the experiment; and to study the parameters of this process, including the percentage of this dangerous pollutant excluded from the body of animals, in relation to the volume of the metal administered. Material and methods. The duration of the experiment was 16 days. There were two groups of animals: experimental and control. All animals were placed in individual cages with identical conditions of stay. Individual Pb doses were administered to every animal in experimental group, depending on the weight of the animal, but in equal daily dose, in mcg per 1 mg of body weight. Results and discussion. The volumes of microelements excreted (per 1 g of animal body weight) were calculated. The first peak of Pb excretion in experimental animal was found on the 5th day of the experiment and comprised 0.7 mg per 1 g of body weight. Then there was a decrease in Pb exclusion, and a gradual increase of this index up to 0.4 mcg for the next 10 days. There was a further significant decrease to the minimum values (0.1 mcg) for 11 days and a re-increase to almost 1.0 mcg for 12 days. The amount of Pb exclusion is mainly affected only by the level of the microelement concentration in the faeces, not the amount of the faeces. In order to determine the percentage of Pb that was excreted, a relative indicator was calculated (where 100% was the amount of Pb that was ingested by each animal (5.9 mg on average). The percentage of microelement removal with faeces from the body of experimental animals did not exceed 2.5%. Two peaks of the highest percentage of Pb excretion were recorded. These were 5 and 12 days, which totally coincided with the maximum concentration of pollutant and the amount of faeces. The correlation with the micronutrient concentration in faeces was extremely high, with r = 0.98. Conclusions. Indicators for the elimination of lead with faeces per day in experimental animals exceeded the same indicators in the control group, which corresponds to the conditions of artificial intake of the metal into the body of the rats in the experimental group. The amount of Pb toxicity per day was more influenced by the concentration of the trace elements in the faeces (in control animals r = 0.90, and in the experimental ones r = 0.98), but not by the amount of faeces. The percentage of excretion of trace elements from the body of experimental animals was low. The relative indicator varied rather broadly throughout the experiment. Some part of the toxicant was excreted from the body of animals with urine, but significant amount of Pb was accumulated in the body.

Keywords: lead, withdrawal, excretion, concentration, correlation

Full text: PDF (Ukr) 254K

References
  1. Bandman AA, Gudzovskyy GA, Dubeykovskaya LS. Vrednye khymycheskye veshchestva. Neorganycheskye soedynenyya elementov I–IV grupp [Harmful chemicals. Inorganic compounds of elements I-IV groups]. Lenyngrad: Khymyya, 1988. 521 p. [Russian]
  2. Vepryuk YuM. Vplyv ksenobiotykiv na funktsiyi nyrok statevonezrilykh ta statevozrilykh shchuriv [The influence of xenobiotics on renal function of the sexually immature and mature rats]. Bukovynskyy medychnyy visnyk. 2009; 13; 4: 53–7. [Ukrainian]
  3. Gygyenycheskye kryteryy sostoyanyya okruzhayushchey sredy. 3. Svynets [Hygienic criteria for the state of the environment. 3. Lead]. Sovmestnoe yzdanye Programmy OON po okruzhayushchey srede y Vsemyrnoy organyzatsyy zdravookhranenyya. Zheneva: VOZ; 1980. 193 p. [Russian]
  4. Dobrovolskyy VV. Geografyya mykroelementov. Globalnoe rasseyanye [The geography of trace elements. Global scattering]. Moskva, 1983. 272 p. [Russian]
  5. Dmytrukha NM, Lugovskyy SP, Lagutina OS. Kharakterystyka imunotoksychnoyi diyi spoluk svyntsyu z mikro- ta nanochastynkamy [Characterization of immunotoxic action of lead compounds with micro- and nanoparticles]. Suchasni problemy toksykologiyi, kharchovoyi ta khimichnoyi bezpeky. 2014; 1–2(64–65): 59–66. [Ukrainian]
  6. Zemlianoy OA. Deyaki osoblyvosti vyvedennya kadmiyu z ekskretsiyamy laboratornykh shchuriv v umovakh eksperymentu [Some features of the removal of cadmium from the excrements of laboratory rats under experimental conditions]. Biologichnyy Visnyk Melitopolskogo derzhavnogo pedagogichnogo universytetu imeni Bogdana Khmelnytskogo. 2014; 4(3): 55–69. [Ukrainian]
  7. Zemlianoy OA. Vplyv svyntsyu na deyaki pokaznyky ekskretsiy laboratornykh shchuriv v umovakh eksperymentu [Lead Effects on Some Excretion Indicators of Laboratory Rats under Experimental Conditions]. Ukrainian journal of medicine, biology and sport. 2018; 3(1/10): 258–63. [Ukrainian] https://doi.org/10.26693/jmbs03.01.258
  8. Lugovskyy SP. Morfo-funktsionalna kharakterystyka golovnogo mozku shchuriv pry khronichnomu vplyvi na organizm malykh doz svyntsyu [Morpho-functional characteristics of rats' brain in case of chronic effects on the body of small doses of lead]. Sovremennye problemy toksykologyy. 2005; 3: 36–43. [Ukrainian]
  9. Pakhomov AE. Sredoobrazuyushchaya funktsyya mlekopytayushchykh kak estestvennaya agrotekhnologyya v pryrodnykh ekosystemakh y ykh yspolzovanye [The environment-forming function of mammals as a natural agrotechnology in natural ecosystems and their use]. Visnyk Dnipropetrovskogo universytetu. Biologiya. 2000; 8(1): 3–8. [Russian]
  10. Trakhtenberg IM, ta in. Porivnyalna kharakterystyka nefrotoksychnykh efektiv rtuti i svyntsyu pry yikh tryvaliy diyi na organizm shchuriv riznogo viku [Comparative characteristic of nephrotoxic effects of mercury and lead in their long-term effects on the body of rats of different ages]. Aktualni problemy transportnoyi medytsyny. 2006; 2: 26–33. [Ukrainian]
  11. Trakhtenberg IM. Knyga pro otruty ta otruyennya. Narysy toksykologiyi [A book on poisons and poisoning. Essays on toxicology]. Per z ros. Ternopil: TDMU; 2008. 364 p. [Ukrainian]
  12. Castellino N, Colicchio G, Rossi A. Folia Med. ... Technicon Symp, New York–London, 1965. p 285-90.
  13. Fioresi M, Simoes MR, Furieri LB, Broseghini-Filho GB, Vescovi MVA, Stefanon I, Vassallo DV. Chronic lead exposure increases blood pressure and myocardial contractility in rats. PLoS One. 2014; 9 (5): e96900. https://www.ncbi.nlm.nih.gov/pmc/articles/4026242. https://doi.org/10.1371/journal.pone.0096900
  14. Wang L, Xun P, Zhao Y, Wang X, Qian L, Chen F. Effects of lead exposure on sperm concentrations and testes weight in male rats: a meta-regression analysis. J Toxicol Environ Health A. 2008; 71 (7): 454–63. https://www.ncbi.nlm.nih.gov/pubmed/18306093. https://doi.org/10.1080/15287390701839331
  15. Gidlow DA. Lead toxicitv. Occupational medicine. 2004; 54: 76−81. https://doi.org/10.1093/occmed/kqh019
  16. Mameli O, Caria MA, Metis F, Solinas A, Tavera C, Ibba A, et al. Neurotoxic effect of lead at low concentrations. Brain Research Bulletin. 2001; 55 (2): 269-75. https://www.ncbi.nlm.nih.gov/pubmed/11470326. https://doi.org/10.1016/S0361-9230(01)00467-1