ISSN 2415-3060 (print), ISSN 2522-4972 (online)
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JMBS 2017, 2(2): 94–101
https://doi.org/10.26693/jmbs02.02.094
Clinical Medicine

Assessment of the Physiological State of the Blood Components using the Method of Registration of a Discharge Luminescence

Pesotskaya L.1, Glukhova H.2, Lakiza T.1, Simonova T.1, Pisarevskaya O.1
Abstract

The relevance of the topic. Existing methods for detection of functional disorders of biological systems or objects today are mostly based on registration of changes of biochemical and chemical quantities. However, these changes appear when already rather pronounced morphological changes in the tissues. Therefore, it is important to study new methods of early detection of functional disorders of various biological objects, based on changes in their biophysical parameters, which are preceded by biochemical changes. It is to register on the photographic material of the glow discharge (GDS) objects, including wildlife, in the field of high voltage, which is known as the Kirlian effect. The aim of the study was to compare the change in G-the glow of lymphocytes and serum of blood in patients with anemia in the addition of various iron preparations. Material and methods. Method of registration of the GRS investigated the serum and blood lymphocytes in 2 patients with iron deficiency anemia in a hospital. Suspensions of lymphocytes and blood serum were isolated using standard laboratory methods. Calculated T-active and cytotoxic T-lymphocytes by the method of rosette, number of erythrocytes in the day of blood sample and in a day of incubation after the addition of different oral iron preparations in comparison with the control. Kirlianography photographing was performed on x-ray film. To register GR-glow of the samples of blood components the experimental device “REK 1” was used developed by Ukrainian research Institute of engineering technologies National mining University (Dnipro). 30 drops were photographed of each sample that was scanned for subsequent computer processing. At the statistical processing of sample images of the corona glow drops of the sample used the average estimate of the number of pixels in a certain sub-band brightness (d). It was calculated the median difference, as the difference in the number of pixels in the current sub-band brightness, in comparison with the previous one. To assess changes in the intensity of illumination in the crown, reflecting its figure, it was calculated the ratio of the differences of median follow-up to the previous one (N+1)/ (N). Resistance of cell membranes to the destruction was the drug Hemineral. For the second patient (P.2) it was less optimal than for P.1, because in addition to hemolysis was caused by lymphocytes. With Globaren at P.1, on the contrary, preserved lymphocytes, but hemolysis was with red blood cells. With Megaterim P.1 all samples presented hemolysis; P. 2 presented hemolysis with lymphocytes, red blood cells, but it is incomplete, with the presence of cell membranes. The differences between the indices of the subbands histograms of GRS samples were correlated with the laboratory data. Method is useful for early detection of damage to biological tissue. Conclusions. GR-glow of blood components varies according to the physiological state of biological samples. To identify the differences and features of GDS of possible biological objects using their histograms of brightness. It is advisable to study the method of registration GRS of biological objects for practical use in medicine.

Keywords: glow discharge, biological object, blood

Full text: PDF (Rus) 3.43M

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